large acelarin logo

A transformation of gemcitabine


Acelarin, our most advanced ProTide, is being evaluated in a global Phase III study for the treatment of patients with advanced biliary tract cancer.

The cytotoxic effect of gemcitabine on cancer cells is attributed to the generation of the triphosphate form of gemcitabine, known as dFdCTP. In cancer cells, dFdCTP interferes with DNA replication causing cancer cell death and preventing tumour growth. However, cancer resistance mechanisms can prevent gemcitabine from generating sufficient levels of dFdCTP inside cancer cells.

Acelarin is comprised of gemcitabine and a phosphoramidate moiety (a phosphate group and a specific combination of aryl, ester and amino acid groups). The addition of the phosphoramidate moiety changes the chemical properties of Acelarin, compared to gemcitabine, resulting in a molecule that can enter the cancer cell independently of nucleoside transporters, is protected from breakdown by deaminases, and, delivers the pre-activated form of gemcitabine.

By overcoming the key cancer resistance mechanisms associated with gemcitabine, Acelarin is able to generate and maintain significantly higher concentrations of the active anti-cancer metabolite (dFdCTP) inside tumour cells.

Gemcitabine (Gemzar®) is used extensively and remains a backbone therapy in the standard of care for the treatment of many cancers, including biliary tract, pancreatic, ovarian, non-small cell lung, bladder and breast cancers. However, resistance to gemcitabine due to its dependence on nucleoside transporters to enter cancer cells, breakdown by deaminases and reliance on cellular kinases for activation can result in a poor survival prognosis for patients.

Acelarin has achieved high response rates and favourable tolerability in patients with advanced solid tumours, including biliary tract cancer.

Number 1 in circle
Enters cancer cells independently of transporters

Gemcitabine requires the human equilibrative transporter (hENT1) to cross the cell membrane. Evidence of this can be seen in assays in which hENT1 was specifically blocked by an inhibitor known as NBTI in human cancer cells. Patients with pancreatic cancer receiving gemcitabine who expressed hENT1 had a median survival of 13 months compared to only 4 months in patients with little or no hENT1 expression. Acelarin does not require hENT1 to enter cells and NBTI had no effect on intracellular levels of the anti-cancer metabolite dFdCTP. This finding was confirmed by blocking hENT1 with dipyridamole, a nucleoside transporter inhibitor, which significantly reduced gemcitabine’s ability to kill cancer cells while Acelarin retained its anti-cancer activity.

Number 2 in circle
Pre-activated, bypassing the need for dCK

Gemcitabine is an inactive, pro-drug that must be phosphorylated, or activated, inside the cell in order to exert a cytotoxic effect. This phosphorylation step requires the enzyme deoxycytidine kinase (dCK) and, for gemcitabine, it is the rate-limiting step in the generation of the anti-cancer metabolite dFdCTP. In patients with pancreatic cancer who were treated with gemcitabine, low levels of dCK were associated with a median overall survival that was 7 months shorter than observed in patients with high levels of dCK. In non-clinical studies, inhibition of dCK by a specific inhibitor, known as 2T2D, significantly reduced the ability of gemcitabine to generate dFdCTP within human cancer cells, whereas Acelarin was unaffected and retained the ability to generate high levels of dFdCTP. Acelarin is pre-phosphorylated and thus does not require dCK for activation. We have confirmed these findings in separate studies in which we blocked dCK with the inhibitor deoxycytidine or by genomic knockdown. In these studies, dCK inactivation significantly reduced the ability of gemcitabine to kill cancer cells while Acelarin retained its activity.

Number 3 in circle
Resistant to breakdown
by CDA

Gemcitabine is susceptible to breakdown by cytidine deaminase (CDA). Gemcitabine-treated patients with high CDA expression had a shorter overall survival compared to those with low CDA expression: 3.9 months vs 8.7 months. In non-clinical studies, Acelarin is protected from breakdown by CDA, while gemcitabine is almost completely degraded in 2 minutes. In addition, resistance to deamination also results in the generation of lower levels of the toxic metabolite dFdU, which is produced when gemcitabine is broken down by CDA.

Spratlin et al, (2004). Clin Cancer Res; 10: 6956-6961     Slusarczyk et al, (2014). J Med Chem; 27: 513-542     Sebastiani et al, (2006). Clin Cancer Res; 12: 2492-2497     Ghazaly et al, (2014). ASCO Poster     Sarr et al, (2019). Scientific Reports; 9: 7643     Bengala et al, (2005). Br J Cancer; 93: 35-40.

Biliary tract cancer


› 340,000

New worldwide cases annually

˜ 18,000

New US cases annually

NuCana aims to replace gemcitabine as the cornerstone of treatment in first-line biliary tract cancer.

Our
Clinical Studies

Acelarin has been investigated in numerous cancer types including biliary tract, ovarian and pancreatic cancers. The lead indication for Acelarin is biliary tract cancer and we are currently conducting a global Phase III registrational study in this indication.

NuTide:121 study logo

Phase III multi-centre study comparing Acelarin + cisplatin to gemcitabine + cisplatin in patients with biliary tract cancer

There are currently no agents approved for the first-line treatment of biliary tract cancer, however, the worldwide standard of care for patients with locally advanced or metastatic disease is the combination of gemcitabine and cisplatin. Patients receiving this regimen have a median overall survival of 11.7 months, compared to 8.1 months for patients receiving gemcitabine as monotherapy.

LARGEST

Enrolling 828 patients making it the largest interventional biliary tract cancer study ever

GLOBAL

Over 130 sites across 15 countries and 4 continents

TRANSFORMATIVE

NuTide:121 aims to change the standard of care for patients with biliary tract cancer

International Study Locations

world map

Acelarin has been granted orphan drug designation for the treatment of biliary tract cancer in both the US and EU.

NuTide:121 is currently enrolling patients with histologically or cytologically confirmed adenocarcinoma of the biliary tract (cholangiocarcinoma, gallbladder, or ampullary cancers) that is locally advanced, unresectable or metastatic, and who have not received prior systemic treatment for biliary tract cancer.

Three interim efficacy analyses, including two designed to support accelerated approval of Acelarin in the US, are planned.

The final analysis will evaluate the OS primary endpoint. It will take place after 637 events have been observed. A statistically significant difference for OS in favour of Acelarin plus cisplatin would be achieved with an improvement in median OS of approximately 2.2 months.

ABC-08

Phase

Ib

PRO-105

Phase

II

ACELARATE

Phase

III

ABC-08 study logo

Phase Ib study of Acelarin in combination with cisplatin in patients with locally advanced or metastatic biliary tract cancer

Patients


14

Evaluable


11

(≥ 1 cycle)

Age


61

(mean)

range 48-78

In this multi-centre, open-label study, Acelarin (625 mg/m2 or 725 mg/m2), was combined with cisplatin (25 mg/m2), on days 1 and 8 of a 21-day cycle. Interim results from the first 14 patients demonstrated an objective response rate (ORR) of 50%. One of the seven patients who achieved a response, had a complete response - a rare occurrence in the treatment of biliary tract cancer. Furthermore, Acelarin was well-tolerated as a first-line treatment for patients with biliary tract cancer.

Previously, the same investigators conducted the ABC-02 clinical study in a similar patient population at many of the same clinical study sites comparing single agent gemcitabine (1000 mg/m2) to the combination of gemcitabine (1000 mg/m2) plus cisplatin (25 mg/m2) and established that gemcitabine plus cisplatin achieved a higher ORR and improved overall survival compared to single-agent gemcitabine. A comparison of the interim ORR data from Cohorts 1 and 2 of the ABC-08 study and ABC-02 study (gemcitabine) is provided in table below:

ABC-08 Study

Acelarin

(625 & 725 mg/m2) + cisplatin

ABC-02 Study

Gemcitabine

(1000 mg/m2) + cisplatin

Complete Response

7% (1/14)

Complete Response

0.6% (1/161)

Partial Response

43% (6/14)

Partial Response

25% (41/161)

Objective Response Rate

50% (7/14)

Objective Response Rate

26% (42/161)

McNamara et al (2018). Ann Oncol; 29: Suppl 8 Abstract ID: TPS544 (ESMO poster 758P  21st Oct, 2018)
Valle et al (2010).  N Eng J Med; 362: 1273-1281
Interim data as of Aug 30, 2018

Acelarin + cisplatin is well-tolerated over multiple cycles with encouraging ORR compared to standard of care.

Tumour volume reductions were durable and all biliary tract cancer subtypes were sensitive to Acelarin in combination with cisplatin.

Safety Profile

  • Acelarin + cisplatin was well-tolerated
  • No unexpected adverse events (AEs)
  • Multiple cycles administered
    (median 8; range 3.5-14)
  • No dose-limiting toxicities (DLTs)
  • Grade 3 AEs included fatigue (21%), neutropaenia (14%), pyrexia (14%), nausea (7%), ALT (14%), AST (7%)
  • No Grade 4 treatment-related AEs
  • No patients discontinued due to Acelarin-related AEs
PRO-105 study logo

Phase II study of Acelarin in patients with platinum-resistant ovarian cancer

This Phase II, dose-finding study of Acelarin enrolled patients with platinum-resistant ovarian cancer who had received at least three prior chemotherapy containing regimens.

Patients with an original diagnosis and/or histological confirmation of high-grade serous, high-grade endometrioid, epithelial ovarian, fallopian tube or primary peritoneal cancer and a platinum-free interval since last line of platinum of less than 6 months were randomised to one of two NUC-1031 doses.

Preliminary data from the 51 patients who participated in Part 1 of the study were announced in March 2020. Patients recruited to Part 1 had received a median of 5 prior lines of therapy and over 70% of them had at least one comorbidity at study entry. Highlighting the fragility of this difficult-to-treat patient population, 45% of patients did not complete the first cycle of treatment with Acelarin. Anti-cancer activity was observed with one patient achieving a complete response, two patients achieving a partial response and 16 patients achieving stable disease with Acelarin. Due to the rapidly evolving treatment landscape and more complex regulatory pathway for single-agent therapy, following the introduction of PARP inhibitors, it was decided not to proceed with Part 2 of the study. Should we elect to pursue any further development of Acelarin in patients with ovarian cancer, we would anticipate combining it with platinum agents.

Acelarate study logo

Phase III study comparing Acelarin with gemcitabine in patients with metastatic pancreatic cancer who are not considered suitable for combination chemotherapy

This study has enrolled 200 patients with metastatic pancreatic cancer. A futility analysis was included in the Acelarate study design to assess the likelihood of the study achieving its primary objective of demonstrating at least a 42% reduction in risk of death compared to gemcitabine. This analysis indicated that this efficacy objective was unlikely to be met in this difficult-to-treat patient population. In particular, 54% of the patients in the Acelarin arm were diagnosed at the most advanced stage T4, compared to 36% of patients in the gemcitabine arm. Recruitment has been suspended to allow the data to mature and conduct additional sub-group analyses. Patients who are deriving benefit can continue treatment with Acelarin.

PRO-002

Phase

Ib

PRO-001

Phase

I

PRO-002 study logo

Phase Ib dose-escalation study of Acelarin in combination carboplatin in patients with recurrent ovarian cancer

Patients


25

Prior lines


3.8

(mean)

range 2-8

Age


64

(mean)

range 37-77

In this Phase Ib study, 25 patients with recurrent ovarian cancer received Acelarin at doses from 500 mg/m2 to 750 mg/m2 in combination with carboplatin (AUC 4 or 5) for a maximum of 6 treatment cycles lasting 3 weeks each. Of the 25 patients enrolled, 23 were evaluable and these patients were characterised by their platinum status: 7 were platinum-refractory, 10 were platinum-resistant, 4 were partially platinum-sensitive and 2 were platinum-sensitive.

The 23 evaluable patients had received 1 or more cycles of Acelarin in combination with carboplatin and had at least 1 radiographic assessment to measure changes in tumour size. Of these patients, 1 achieved a complete response and 8 achieved a partial response, although not all of these patients have received a confirmatory scan. This resulted in an overall response rate of 39%. Another 13 patients achieved stable disease, resulting in a disease control rate of 96%.

The median progression-free survival duration for all 25 patients recruited was 7.3 months.

Safety Profile

  • Acelarin + carboplatin was well-tolerated
  • No unexpected adverse events (AEs)
  • No thrombocytopaenia in the platinum-partially sensitive or sensitive patients (n=7)
  • 6 dose-limiting toxicities (DLTs) in 4 patients:

    • 2 Grade 4 thrombocytopaenia
    (Acelarin 625 mg/m2 & 750 mg/m2 + carboplatin AUC4)
    • 3 Grade 3 fatigue
    (Acelarin 625 mg/m2 + carboplatin AUC4)
    • 1 Grade 4 neutropaenia
    (Acelarin 750 mg/m2 + carboplatin AUC4)

PRO-001 study logo

Phase I dose-escalation study of Acelarin in patients with advanced solid tumours

Patients


68

Prior lines


3

(mean)

range 1-10

Age


56

(mean)

range 20-83

In this Phase I study, 68 patients with rapidly progressing advanced solid tumours and metastatic disease that had exhausted all standard treatment options received Acelarin at doses ranging from 375 mg/m2 to 1000 mg/m2.

A subset of 49 patients were considered evaluable having received two or more cycles of Acelarin, equivalent to two or more months of therapy, and had at least one follow-up radiographic assessment to measure changes in tumour size. Of these patients, 5 achieved a partial response, although not all of these patients received a final confirmatory scan. Another 33 patients had stable disease, resulting in an overall disease control rate of 78% in the evaluable population.

The mean progression-free survival duration for patients achieving partial response or stable disease was 6.7 months (range 2–24 months).

Safety Profile

  • Acelarin was well-tolerated
  • Most common AEs* Grade 1 or 2 were:
    transaminitis, fatigue, decreased WBC, thrombocytopaenia
  • 26 Serious adverse events*
  • 5 patients had Grade 4 AEs*:
    neutropaenia, thrombocytopaenia, sepsis; raised GGT, dyspnoea, posterior reversible encephalopathy syndrome, hypomagnesaemia
  • 4 dose-limiting toxicities (DLTs) were observed:

    • Grade 3 elevated ALT (725 mg/m2 & 1000 mg/m2)
    • Grade 4 thrombocytopaenia (750 mg/m2 & 1000 mg/m2)

* Considered definitely, probably or possibly related to Acelarin